Thanks for the feedback everybody.
To answer some of the questions:
This was taken with a Tamron 90mm macro lens - no extension tubes used. The shooting distance was probably about 2 or 3 cm - it was very close. The spikes are actually only about 5mm long - it's a really tiny cactus.
Some of the light fall-off was natural. This was taken looking down on the cactus, and it was positioned so that the focal point of the shot (the main cactus 'stem') was in soft light, whereas the rest of the plant was in slightly darker light. I accentuated this slightly in Photoshop using the burn tool.
The reason I entered this and the other one was because, although they were of the same cactus, I thought they were really quite different. This photo was to concentrate on the shape and form of the spikes - the other shot was all about the water drops, and how they 'held' within all the spines.
Thanks again for the feedback.